Oxygen and pH regulation of protein synthesis in mitochondria from Artemia franciscana embryos

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Oxygen and pH regulation of protein synthesis in mitochondria from Artemia franciscana embryos.

To identify factors responsible for the down-regulation of mitochondrial biosynthetic processes during anoxia in encysted Artemia franciscana embryos, the effects of oxygen limitation and pH on protein synthesis were investigated in isolated mitochondria. At the optimal pH of 7.5, exposure of mitochondria to anoxia decreases the protein synthesis rate by 79%. Rates were suppressed by a further ...

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Regulatory features of transcription in isolated mitochondria from Artemia franciscana embryos.

Optimal conditions were developed for an in organello transcriptional run-on assay using mitochondria isolated from Artemia franciscana embryos to investigate potential regulatory features of RNA synthesis under conditions of anoxia-induced quiescence. Transcription is not dependent on oxidative phosphorylation for maximal activity when exogenous ATP is available. Bona fide transcription produc...

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synthesis of sulfides from alcohols and thiols in solvent-freeconditions and deoxygenation of sulfoxides

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Regulation of promoter occupancy during activation of cryptobiotic embryos from the crustacean Artemia franciscana.

Artemia franciscana embryos can suspend their development and metabolism at the gastrula stage to enter a state of cryptobiosis, forming cysts. Embryonic development and metabolism can be resumed under favorable environmental conditions to give rise to free-swimming larvae or nauplii. The mechanisms that mediate these processes are not completely known. Here, we report our studies of the mechan...

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Quiescence in Artemia franciscana embryos: reversible arrest of metabolism and gene expression at low oxygen levels.

Depression of the production and consumption of cellular energy appears to be a prerequisite for the survival of prolonged bouts of anoxia. A correlation exists between the degree of metabolic depression under anoxia and the duration of anoxia tolerance. In the case of brine shrimp (Artemia franciscana) embryos, oxygen deprivation induces a reversible quiescent state that can be tolerated for s...

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ژورنال

عنوان ژورنال: Biochemical Journal

سال: 1996

ISSN: 0264-6021,1470-8728

DOI: 10.1042/bj3130207